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Influenza Virus A H1N1, Molecular Detection

Molecular screening for the presence of type A influenza viruses, subtype H1N1, is used to quickly and accurately confirm laboratory confirmation of infection by the specific subtype of influenza virus. This screening is useful for timely documentation of influenza, which allows for the rapid application of appropriate treatment and prophylaxis. This subtype has recently caused (2010) influenza epidemics with several deaths. This flu is known as swine flu.

Influenza virus, the causative agents of influenza, are RNA viruses of the Orthomyxoviridae family. The genome of influenza viruses is fragmented and comprises eight separate RNA clones, each encoding a specific protein. The virus is surrounded by an envelope consisting of lipids of the cell membrane together with viral proteins (hemagglutinin and neuraminidase, responsible for viral infectivity and virus release). Influenza viruses are genetically altered. Minor antigenic changes are the general rule (antigenic deflection) and are explained by the natural selection of point mutations in the hemagglutinin, under the pressure of the immune system. More pronounced changes (antigenic shifts) explain the periodic occurrence of influenza epidemics and pandemics.

Influenza viruses are airborne and multiply normally in the nasopharynx mucosa, resulting in the appearance of rhinitis, pharyngitis or tracheobronchitis, after a 24-72 hour incubation period. Pneumonia caused solely by the influenza virus is rare. As a rule, pneumonia is caused by infections with Staphylococcus, Streptococcus, Pneumococcal or Hemophilic. These infections can be controlled with antibiotics.

Type A flu virus is the most important and most frequently observed influenza virus. It causes repeated epidemics or even pandemics from time to time. After a 1-5 day incubation period in which the viruses multiply in the nasopharynx mucosa, rhinitis and pharyngitis accompanied by fever develop. Headache and muscle pain (myositis) may occur, which may be accompanied by nausea and loss of appetite. After 6 days, the fever may fall, but rhinitis, cough, and weakness persist for at least 1-2 weeks.

Type B influenza virus tends to have less antigenic changes and is involved in only a few epidemic outbreaks.




Important Note

Laboratory test results are the most important parameter for the diagnosis and monitoring of all pathological conditions. 70%-80% of diagnostic decisions are based on laboratory tests. Correct interpretation of laboratory results allows a doctor to distinguish "healthy" from "diseased".

Laboratory test results should not be interpreted from the numerical result of a single analysis. Test results should be interpreted in relation to each individual case and family history, clinical findings and the results of other laboratory tests and information. Your personal physician should explain the importance of your test results.

At Diagnostiki Athinon we answer any questions you may have about the test you perform in our laboratory and we contact your doctor to get the best possible medical care.



Nasal Swab

Nasal Aspirate

Pharyngeal Swab


Bronchoalveolar Lavage (BAL)


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