The measurement of methadone in serum is critical for monitoring the therapeutic levels of the drug, ensuring that the dosage is effective yet safe, and avoiding underdosing or potentially toxic levels. The test can also be used to confirm adherence to a treatment regimen or to diagnose suspected overdose.
Methadone is a synthetic opioid primarily used for opioid dependency treatment and as an analgesic. When methadone is administered, it is metabolized by the liver and distributed throughout the body, including into the plasma.
Plasma levels are directly related to drug efficacy and safety, influencing clinical decisions regarding dose adjustments.
Methadone is measured through liquid chromatography coupled with mass spectrometry (LC-MS/MS), a sensitive and specific method that allows for the precise quantification of methadone in plasma.
